| 货号 | AF2205-SP |
| 别名 | AXCAM; axonal-associated cell adhesion molecule; BIG-2; BIG-2Brain-derived immunoglobulin superfamily protein 2; CNTN4; contactin 4; contactin-4; MGC33615; neural cell adhesion protein BIG-2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects Contactin‑4 isoform a in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CNTN2, recombinant mouse CNTN3, and rhCNTN5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: The enhancement of neurite outgrowth from E16-18 rat hippocampal neurons induced by rhCNTN-4 (400 ng/3 μL, drop/well) is significantly inhibited by the antibody at a concentration of 5 μg/mL. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 152330 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. BIG-2 mediates olfactory axon convergence to target glomeruli. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Contactin-4 isoform a Asp19-Gly1001 Accession # Q8IWV2 |
| 内毒素水平 | <0.25 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Contactin-4 (CNTN4), also known as BIG-2 (Brain-derived Immunoglobulin Superfamily molecule 2), is an axonal cell adhesion molecule that belongs to the contactin family, a subfamily of the Ig superfamily (1‑3). The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) and are characterized by the presence of six Ig-like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)‑anchoring domain. Contactin family proteins exist as membrane-bound proteins, but can also be released as soluble proteins by GPI‑specific phospholipase D. Soluble Contactins are functionsl proteins that are able to promote neurite outgrowth. Human CNTN4 has been mapped to chromosome 3p26‑p25. Three alternative transcripts of CNTN4, encoding isoforms a, b, and c precursor proteins containing 1026, 282, and 698 amino acid (aa) residues, respectively, have been described. Human CNTN4 isoform a shares 94% aa sequence identity with its rat homolog. It also shares from 44‑66% aa sequence identity with other CNTN family members. CNTN family members display overlapping but distinct expression patterns. CNTN4 expression is detected in multiple organs including brains, pancreas, kidney, aorta, small intestine, thyroid, uterus and testis. However, expression of the 282 aa isoform b is primarily restricted to the brain. CNTN4 has been suggested to play important roles in the formation of neuronal networks during nervous sytems development. Disruption of CNTN4 has been implicated in the 3p deletion syndrome characterized by growth failure, developmental delay, and mental retardation. CNTN-4 expression is induced in human neuroblastoma tumor cells treated with retinoic acid and may be responsible for the the neuritogenic response of tumor cells to retinoids. R&D Systems recombinant CNTN4 shows in vitro neurite outgrowth promoting activity when used as an immobilized substrate for hippocampal neuron outgrowth. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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