Human IL-10 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-10 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-217-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-217-SP
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Human
应用	Western Blot,Simple Western,Immunohistochemistry,Neutralization
目标/特异性	Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant Epstein-Barr virus IL-10 is observed and less than 20% cross-reactivity with recombinant mouse IL‑10, recombinant feline IL-10, recombinant porcine IL‑10, recombinant equine IL-10, and recombinant rat IL‑10 is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Role of Interleukin-10 on Nasal Polypogenesis in Patients with Chronic Rhinosinusitis with Nasal Polyps PLoS ONE, 2016;11(9):e0161013. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F Leukemia, 2016;30(1):163-72. Species: Human Sample Type: Whole Cells Application: Neut Expression of Tim-1 in primary CNS lymphoma Cancer Med, 2016;5(11):3235-3245. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded Immune suppression and resistance mediated by constitutive activation of Wnt/beta-catenin signaling in human melanoma cells. J. Immunol., 2012;189(5):2110-7. Species: Human Sample Type: Whole Cells Application: Neut IL-10 expression by primary tumor cells correlates with melanoma progression from radial to vertical growth phase and development of metastatic competence. Authors: Itakura E, Huang RR, Wen DR, Paul E, Wunsch PH, Cochran AJ Mod. Pathol., 2011;24(6):801-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype. Authors: Ouwehand K, Oosterhoff D, Breetveld M J. Invest. Dermatol., 2011;131(2):418-25. Species: Human Sample Type: Whole Tissue Application: Neut Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Blood, 2010;116(12):2061-9. Species: Human Sample Type: Whole Tissue Application: IHC - Not specified Evolving immunosuppressive microenvironment during human cervical carcinogenesis. Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K Mucosal Immunol, 2008;1(5):412-20. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Eur. J. Immunol., 2008;38(4):1012-23. Species: Human Sample Type: Whole Cells Application: Neut T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue. Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC Blood, 2007;109(11):4856-64. Species: Human Sample Type: Whole Cells Application: Neut Leukocyte-derived IL-10 reduces subepithelial fibrosis associated with chronically inhaled endotoxin. Authors: Garantziotis S, Brass DM, Savov J, Hollingsworth JW, McElvania-TeKippe E, Berman K, Walker JK, Schwartz DA Am. J. Respir. Cell Mol. Biol., 2006;35(6):662-7. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk. Authors: Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de La Pintiere CT, Genetet N, Patard JJ, Catros-Quemener V Clin. Exp. Immunol., 2006;146(3):518-23. Species: Human Sample Type: Whole Tissue Application: IHC Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma. Authors: Pound JD, Batth BK, Johannessen I, Wood K J. Immunol., 2005;174(5):3015-23. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates. Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ J. Immunol., 2004;172(9):5753-64. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10 Ser19-Asn178 Accession # P22301
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, IL-24, and IL-26/AK155 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL-10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (11‑13). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).
运输条件	Blue Ice
存放说明	4℃
参考文献	Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172. Hsu, D.-H. et al. (1990) Science 250:830. Windsor, W.T. et al. (1993) Biochemistry 32:8807. Syto, R. et al. (1998) Biochemistry 37:16943. Kotenko, S.V. et al. (1997) EMBO J. 16:5894. Kotenko, S.V.et al. (2000) J. Biol. Chem. 276:2725. Hor, S. et al. (2004) J. Biol. Chem. 279:33343. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

参考图片

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).
A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑10 in Human Tonsil.

IL‑10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL‑10 in Human Tonsil. IL‑10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑217‑NA) at 15 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human IL‑10 by Simple Western^TM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 at approximately 23 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody.

Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA). The ND₅₀ is typically 0.2-1.0 µg/mL.