Human IL-13 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-13 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-213-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-213-SP
别名	ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
全称	Interleukin 13
反应种属	Human
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry,Neutralization
目标/特异性	Detects human IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross‑reactivity with recombinant rhesus monkey IL-13 is observed, approximately 25% cross‑reactivity with recombinant canine IL-13 is observed, and approximately 15% cross‑reactivity with recombinant rat IL-13 is observed and less than 5% cross‑reactivity with recombinant mouse IL-13 and recombinant cotton rat IL-13 is observed.
使用方法	Western Blot: 0.1 µg/mL CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Intracellular Staining by Flow Cytometry: 5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.5-3 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Eur. J. Immunol., 2008;38(4):1012-23. Species: Human Sample Type: Whole Cells Application: Neut Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns. Authors: Rhoton-Vlasak A, Hardt N Fertil. Steril., 2005;83(3):659-65. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas. Authors: Ripley D, Shoup B, Majewski A Gynecol. Oncol., 2004;92(3):761-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas. Authors: Ripley D, Shoup B, Majewski A Gynecol. Oncol., 2004;92(3):761-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Human lung myofibroblasts as effectors of the inflammatory process: the common receptor gamma chain is induced by Th2 cytokines, and CD40 ligand is induced by lipopolysaccharide, thrombin and TNF-alpha. Authors: Doucet C, Giron-Michel J, Canonica GW, Azzarone B Eur. J. Immunol., 2002;32(9):2437-49. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-13 Gly21-Asn132 Accession # AAK53823
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus macaque IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
运输条件	Blue Ice
存放说明	4℃
参考文献	Wills-Karp, M. (2004) Immunol. Rev. 202:175. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231. Ruetten, H. and C. Thiemermann (1997) Shock 8:409. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464. Tabata, Y. et al. (2007) J. Immunol. 177:7905. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

参考图片

Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody. Recombinant Human IL‑13 (Catalog # 213-ILB) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA). The ND₅₀ is typically 0.5-3 µg/mL.