| 货号 | AF2127-SP |
| 别名 | EC 3.4.21; FLJ31863; KREMEM1; kremen protein 1; KREMEN; kringle containing transmembrane protein 1; kringle containing transmembrane protein; Kringle domain-containing transmembrane protein 1; kringle-coding gene marking the eye and the nose; Kringle-containing protein marking the eye and the nose; KRM1; KRM1Dickkopf receptor | 全称 | Kringle-containing Transmembrane Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Kremen-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kremen-1 and less than 5% cross-reactivity with recombinant human Kremen-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 83999 (Human); 84035 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Kremen‑1 Ala20-Gly397 Accession # NP_114434 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Kremen-1 is a type I transmembrane protein that contains extracellular Kringle, WSC, and CUB domains. It is expressed in diverse embryonic and adult tissues. Kremen proteins bind the secreted Dickkopf proteins Dkk-1, -2, and -4 with high affinity to antagonize the canonical Wnt signaling pathway. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Kremen‑1 in Human Colon Cancer Tissue. Kremen‑1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Kremen‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Kremen‑1 in Human Colon. Kremen‑1 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human Kremen‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
