Human TNF-alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human TNF-alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF-210-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-210-SP
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Human
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects human TNF‑ alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant feline TNF‑ alpha, recombinant canine TNF‑ alpha, recombinant rhesus monkey TNF‑ alpha, and recombinant equine TNF-alpha is observed and approximately 15% cross‑reactivity with recombinant cotton rat TNF‑ alpha, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant guinea pig TNF‑ alpha is observed and less than 5% cross‑reactivity with recombinant porcine TNF‑ alpha and recombinant bovine TNF‑ alpha is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.01-0.06 μg/ml in the presence of 0.75 ng/mL Recombinant Human TNF‑ alpha.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients PLoS ONE, 2016;11(9):e0162908. Species: Human Sample Type: Whole Cells Application: Bioassay Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT. Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G Proc Natl Acad Sci U S A, 2014;111(52):E5688-96. Species: Human Sample Type: Whole Cells Application: Neut Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Authors: Todd J, Simpson P, Estis J, Torres V, Wub A Cytokine, 2013;64(3):660-5. Species: Human Sample Type: Plasma Application: ELISA Development The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P J. Immunol., 2011;186(7):4175-82. Species: Human Sample Type: Whole Cells Application: Neut Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein. Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL Cytokine, 2011;53(1):74-83. Species: Human Sample Type: Cell Lysates Application: WB Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Blood, 2010;116(12):2061-9. Species: Human Sample Type: Whole Tissue Application: IHC - Not specified Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohns disease. Authors: Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW J. Exp. Med., 2009;206(9):1883-97. Species: Human Sample Type: Cell Lysates Application: WB KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis. Authors: Navenot JM, Fujii N, Peiper SC Mol. Pharmacol., 2009;75(5):1074-83. Species: Human Sample Type: Whole Cells Application: Neut Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Authors: Skibinski G, Elborn JS, Ennis M Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76. Species: Human Sample Type: Whole Cells Application: Neut Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1. Authors: Lee SY, Cherla RP, Tesh VL Infect. Immun., 2007;75(3):1291-302. Species: Human Sample Type: Whole Cells Application: Functional Assay Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways. Authors: Hunter I, Nixon GF J. Biol. Chem., 2006;281(45):34705-15. Species: Human Sample Type: Whole Cells Application: Neut Sepsis and pathophysiology of anthrax in a nonhuman primate model. Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S Am. J. Pathol., 2006;169(2):433-44. Species: Primate - Papio cynocephalus (Yellow Baboon) Sample Type: Plasma Application: ELISA Development Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104. Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D J. Leukoc. Biol., 2005;78(2):359-71. Species: Human Sample Type: Whole Cells Application: Neut Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis. Authors: Andersson By U, Tani E, Andersson U J. Pediatr. Hematol. Oncol., 2004;26(11):706-11. Species: Human Sample Type: Whole Cells Application: ICC Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo. Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE Photochem. Photobiol., 2004;80(2):231-5. Species: Human Sample Type: Whole Tissue Application: IHC Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta. Authors: Park JS, Kim YS, Jee YK, Myong NH, Lee KY Scand. J. Immunol., 2003;57(5):463-9. Species: Human Sample Type: Whole Cells Application: Neut Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells. Authors: Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ Eur. J. Immunol., 2002;32(5):1464-71. Species: Human Sample Type: Cell Lysates Application: WB Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1. Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD J Leukoc Biol, 0;91(2):299-309. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human TNF-alpha Val77-Leu233 Accession # P01375
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types. Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%‑92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-alpha regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-alpha is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cancer Biomarkers Conventional/Classical Dendritic Cells Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction gamma delta T Cells Inflammatory Mediators Inflammatory/Monocyte-derived Dendritic Cells Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Molecules Secreted by VSMC Natural Killer T (NKT) Cells Neuroinflammation Obesity Plasmacytoid Dendritic Cells Preeclampsia Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members T Cell Cytokine Signaling Th1 Cells Th22 Cells TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF-alpha Antibody.

Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND₅₀ is typically 0.01‑0.06 μg/mL.

TNF‑ alpha in Human PBMCs. TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-210-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.