Human CXCL8/IL-8 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CXCL8/IL-8 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-208-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-208-SP
别名	3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
全称	Interleukin 8
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunocytochemistry,Neutralization
目标/特异性	Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant canine IL-8/CXCL8, recombinant feline IL-8/CXCL8 and recombinant porcine IL-8/CXCL8 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.1-0.5 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Necrotic cells influence migration and invasion of glioblastoma via NF-?B/AP-1-mediated IL-8 regulation Authors: SH Ahn, H Park, YH Ahn, S Kim, MS Cho, JL Kang, YH Choi Sci Rep, 2016;6(0):24552. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The presence of a matrix-derived neutrophil chemoattractant in bronchiolitis obliterans syndrome after lung transplantation. Authors: Hardison MT, Galin FS, Calderon CE, Djekic UV, Parker SB, Wille KM, Jackson PL, Oster RA, Young KR, Blalock JE, Gaggar A J. Immunol., 2009;182(7):4423-31. Species: Human Sample Type: Whole Cells Application: Neut Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis. Authors: Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M J. Invest. Dermatol., 2009;129(3):650-6. Species: Mouse Sample Type: In Vivo Application: In Vivo Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo. Authors: Bendrik C, Dabrosin C J. Immunol., 2009;182(1):371-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo. Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE Photochem. Photobiol., 2004;80(2):231-5. Species: Human Sample Type: Whole Tissue Application: IHC Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation. Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS J. Virol., 2003;77(3):1727-37. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Cells Application: Neut Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM J. Immunol., 2002;169(11):6515-21. Species: Human Sample Type: BALF Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-8/CXCL8 Ser28-Ser99 Accession # P10145
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivoandin vitro.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cancer Biomarkers Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors Diabetes HIF Transcription Factors Infectious Diseases Inflammatory Mediators Macrophage Activation Markers Megakaryocytes Molecules Secreted by VSMC Neuroinflammation Platelets

参考图片

IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑208‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL-8/CXCL8 in Human Tonsil.
IL-8/CXCL8 was detected in immersion fixed paraffin-embedded sections of human tonsil (surgically removed due to severe EBV-induced mononucleosis) using Human
IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑208‑NA) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Chemotaxis Induced by
IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody.

Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND₅₀ is typically
0.1‑0.5 µg/mL.