Human/Mouse Brachyury Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse Brachyury Affinity Purified Polyclonal Ab (25 UG)

货号： AF2085-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2085-SP
别名	brachyury protein; MGC104817; Protein T; T brachyury (mouse) homolog; T brachyury homolog; T, brachyury homolog (mouse); TF; TFT
反应种属	Human/Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Chromatin Immunoprecipitation (ChIP): Brachyury/DNA immunocomplexes were detected in sheared chromatin from fixed BG01V Human embryonic stem cells using standard PCR for the VEGF promoter sequence. Use 5 μg of antibody per 5x10⁶cells. Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6862 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation Stem Cell Res, 2016;17(2):222-227. Species: Human Sample Type: Whole Cells Application: IHC Not Specified Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells PLoS ONE, 2016;11(7):e0159246. Species: Mouse Sample Type: Whole Tissue Application: IHC - Fresh PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Authors: Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W Nat Commun, 2015;6(0):7212. Species: Human Sample Type: Cell Lysates Application: WB Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation. Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T Development, 2015;142(9):1628-38. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen SRF is essential for mesodermal cell migration during elongation of the embryonic body axis. Authors: Schwartz B, Marks M, Wittler L, Werber M, Wahrisch S, Nordheim A, Herrmann B, Grote P Mech Dev, 2014;133(0):23-35. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function. Authors: Fang F, Wasserman S, Torres-Vazquez J, Weinstein B, Cao F, Li Z, Wilson K, Yue W, Wu J, Xie X, Pei X J Cell Sci, 2014;127(0):1428-40. Species: Human Sample Type: Whole Cells Application: IF T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness. Authors: Pinto F, Pertega-Gomes N, Pereira M, Vizcaino J, Monteiro P, Henrique R, Baltazar F, Andrade R, Reis R Clin Cancer Res, 2014;20(18):4949-61. Species: Human Sample Type: Cell Lysates Application: WB In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. Authors: Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James PLoS Biol, 2014;12(8):e1001937. Species: Human Sample Type: Whole Cells Application: IF The endothelial cell line bEnd.3 maintains human pluripotent stem cells. Authors: Joubin, Katherin, Richardson, Amelia, Novoa, Natalia, Tu, Edmund, Tomishima, Mark J Stem Cells Dev, 2012;21(12):2312-21. Species: Human Sample Type: Whole Cells Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cells. Authors: Evans AL, Faial T, Gilchrist MJ PLoS ONE, 2012;7(3):e33346. Species: Human Sample Type: Cell Lysates Application: ChIP A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ J. Cell. Sci., 2011;124(0):1992-2000. Species: Human Sample Type: Whole Cells Application: ICC Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M Cell Stem Cell, 2009;4(3):248-62. Species: Human Sample Type: Whole Cells Application: ICC A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P Dev. Biol., 2009;330(2):286-304. Species: Mouse Sample Type: Whole Cells Application: ICC TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL Nat. Cell Biol., 2008;10(7):837-48. Species: Human Sample Type: Whole Cells Application: ICC Creation of engineered human embryonic stem cell lines using phiC31 integrase. Authors: Thyagarajan B, Liu Y, Shin S, Lakshmipathy U, Scheyhing K, Xue H, Ellerstrom C, Strehl R, Hyllner J, Rao MS, Chesnut JD Stem Cells, 2007;26(1):119-26. Species: Human Sample Type: Whole Cells Application: ICC Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking. Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD Biotechnol. Bioeng., 2007;97(6):1617-25. Species: Human Sample Type: Cell Lysates Application: WB Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Authors: McLean AB, D&amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Stem Cells, 2007;25(1):29-38. Species: Human Sample Type: Cell Lysates Application: WB Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Authors: Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H Blood, 2007;111(4):1933-41. Species: Human Sample Type: Whole Cells Application: ICC A porous photocurable elastomer for cell encapsulation and culture. Authors: Gerecht S, Townsend SA, Pressler H, Zhu H, Nijst CL, Bruggeman JP, Nichol JW, Langer R Biomaterials, 2007;28(32):4826-35. Species: Human Sample Type: Whole Cells Application: ICC Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH FEBS Lett., 2006;580(25):5869-74. Species: Human Sample Type: Whole Cells Application: ICC Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Brachyury Ser2-Glu202 Accession # O15178
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Transcription Factors Early Mesodermal Lineage Markers Hemangioblast Transcription Factors Hemangioblasts ICC / IHC Images: Brachyury Pluripotent Stem Cell Transcription Factors Transcription Factors and Regulators

参考图片

Brachyury in Differentiated Human Embryonic Stem Cells. Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Brachyury in BG01V Human Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Brachyury in Embryonic Mouse Notochord. Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Brachyury-regulated Genes by Chromatin Immunoprecipitation. Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.