| 货号 | AF2084-SP |
| 别名 | angiopoietin-like 2; Angiopoietin-like protein 2; angiopoietin-related protein 2; ANGPTL2; ANGRP2; ARP2HARP; MGC8889 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human Angiopoietin-like 2 (ANGPTL2) in direct ELISAs and Western blots. In these formats, approximately 60% cross-reactivity with recombinant mouse ANGPTL2 is observed and less than 1% cross-reactivity with recombinant human (rh) Ang-1 and rhAng-2 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23452 (Human); 26360 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 2 Gln23-Phe492 Accession # Q9UKU9 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ANGPTL2, also known as angiopoietin-related protein 2 (ARP2), contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, which are structurally conserved in angiopoietins. Unlike angiopoietins which are ligands for TIE2, ANGPTL2 is an orphan ligand that does not bind TIE2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot. Western blot shows lysates of human heart tissue, human aorta tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 55-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
