Human IL-7 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-7 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-207-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-207-SP
别名	IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
全称	Interleukin 7
反应种属	Human
应用	Western Blot,Neutralization
目标/特异性	Detects human IL-7 in direct ELISAs and Western blots. In Western blots, detects recombinant mouse IL-7 but no cross-reactivity with recombinant rat IL-17.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND₅₀) is typically 0.02-0.04 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑7.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3574 (Human); 16196 (Mouse); 25647 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin 7 plays a role in T lymphocyte apoptosis inhibition driven by mesenchymal stem cell without favoring proliferation and cytokines secretion. Authors: Normanton M, Alvarenga H, Hamerschlak N, Ribeiro A, Kondo A, Rizzo L, Marti L PLoS ONE, 2014;9(9):e106673. Species: Human Sample Type: Whole Cells Application: Neut Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism. Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D J. Immunol., 2005;174(4):2376-86. Species: Human Sample Type: Whole Cells Application: Neut Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. Authors: Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW J. Immunol., 2005;175(11):7325-31. Species: Human Sample Type: Whole Cells Application: Neut Structure function analysis of interleukin 7: requirement for an aromatic ring at position 143 of helix D. Authors: vanderSpek JC, Sutherland JA, Gill BM, Gorgun G, Foss FM, Murphy JR Cytokine, 2002;17(5):227-33. Species: Human Sample Type: Cell Culture Supernates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-7 Asp26-His177 Accession # P13232
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺ and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity. IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Lymphoblastic Leukemia Common gamma Chain Receptor Family Isolation, Culture, and Identification of Natural Killer Cells Multiple Myeloma Natural Killer T (NKT) Cells NCR-ILC3 Platelets Th2 Cells

参考图片

Detection of Recombinant Human and Mouse IL‑7 by Western Blot. Western blot shows 25 ng of Recombinant Human IL‑7 (Catalog # 207-IL), Recombinant Mouse IL‑7 (Catalog # 407-ML) and Recombinant Rat IL‑7 (Catalog # 7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL‑7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND₅₀ is typically 0.02-0.04 µg/mL.