| 货号 | AF2070-SP |
| 别名 | GASP1; GASP-1; Growth and differentiation factor-associated serum protein 1; hGASP-1; WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein; WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2; WAP, FS, Ig, two KU and NTR module related protein; WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2; WFIKKN2; WFIKKN-related protein; WFIKKNRP; WFIKKNRPmultivalent protease inhibitor protein | 全称 | Growth and Differentiation Factor-associated Serum Protein-1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human GASP‑1/WFIKKNRP in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human GASP-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human GASP-1 (1.5 µg/mL, Catalog # 2070-GS) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (5 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 18.9 µg/mL. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 124857 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human GASP‑1/WFIKKNRP Leu35-His576 Accession # Q8TEU8 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKNRP. WAP, follistatin, Kazal and netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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