Human IL-6 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-6 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-206-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-206-SP
别名	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
全称	Interleukin 6
反应种属	Human
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
使用方法	Western Blot: 0.2 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically 15-45 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ Authors: M Sameni, D Cavallo-Me, OE Franco, A Chalasani, K Ji, N Aggarwal, A Anbalagan, X Chen, RR Mattingly, SW Hayward, BF Sloane Breast Cancer Res., 2017;19(1):56. Species: Human Sample Type: Whole Cells Application: Neut SorLA in interleukin-6 signaling and turnover Authors: JV Larsen, CM Petersen Mol. Cell. Biol, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: ICC Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation. Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G Nat Cell Biol, 2015;17(9):1193-204. Species: Human Sample Type: Cell Lysates Application: WB Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration. Authors: Osuala K, Sameni M, Shah S, Aggarwal N, Simonait M, Franco O, Hong Y, Hayward S, Behbod F, Mattingly R, Sloane B BMC Cancer, 2015;15(0):584. Species: Human Sample Type: Whole Cells Application: Neut Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects. Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D J Immunol Res, 2014;2014(0):593562. Species: Human Sample Type: Whole Cells Application: Functional Assay Digital microfluidic assay for protein detection. Authors: Mok J, Mindrinos M, Davis R, Javanmard M Proc Natl Acad Sci U S A, 2014;111(6):2110-5. Species: Human Sample Type: Recombinant Protein Application: Microfluidic Assay Inhibition of IL-6 expression in LNCaP prostate cancer cells by a combination of atorvastatin and celecoxib. Authors: Wang H, Cui X, Goodin S, Ding N, Van Doren J, Du Z, Huang M, Liu Y, Cheng X, Dipaola R, Conney A, Zheng X Oncol Rep, 2014;31(2):835-41. Species: Human Sample Type: Whole Cells Application: ICC Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype. Authors: Freund A, Patil CK, Campisi J EMBO J., 2011;30(8):1536-48. Species: Human Sample Type: Whole Cells Application: ICC Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS. Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C J. Biol. Chem., 2011;286(39):34271-85. Species: Human Sample Type: Cell Culture Supernates Application: WB Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA Cancer Res., 2009;69(1):329-37. Species: Human Sample Type: Whole Cells Application: ICC Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha. Authors: Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A Endocrinology, 2008;149(4):1678-86. Species: Human Sample Type: Cell Lysates Application: WB Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production. Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE Proc. Natl. Acad. Sci. U.S.A., 2008;105(38):14692-7. Species: Human Sample Type: Whole Cells Application: Neut Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network. Authors: Kuilman T, Michaloglou C, Vredeveld LC, Douma S, van Doorn R, Desmet CJ, Aarden LA, Mooi WJ, Peeper DS Cell, 2008;133(6):1019-31. Species: Human Sample Type: Cell Lysates Application: WB T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue. Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC Blood, 2007;109(11):4856-64. Species: Human Sample Type: Whole Cells Application: Neut Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Authors: Skibinski G, Elborn JS, Ennis M Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk. Authors: Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de La Pintiere CT, Genetet N, Patard JJ, Catros-Quemener V Clin. Exp. Immunol., 2006;146(3):518-23. Species: Human Sample Type: Whole Tissue Application: IHC Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS Exp. Cell Res., 2006;312(10):1909-23. Species: Human Sample Type: Whole Cells Application: Neut Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkins lymphoma. Authors: Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Duhrsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U Clin. Cancer Res., 2006;12(15):4491-8. Species: Human Sample Type: Whole Cells Application: Neut Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo. Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE Photochem. Photobiol., 2004;80(2):231-5. Species: Human Sample Type: Whole Tissue Application: IHC Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation. Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS J. Virol., 2003;77(3):1727-37. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Cells Application: Neut Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey. Authors: Sengupta J, Dhawan L, Ghosh D Cytokine, 2003;24(6):277-85. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-6 Pro29-Met212 Accession # P05231
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6-9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Van Snick, J. (1990) Annu. Rev. Immunol. 8:253. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502. Jones, S.A. (2005) J. Immunol. 175:3468. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227. Hirano, T. et al. (1986) Nature 324:73. Alberti, L. et al. (2005) Cancer Res. 65:2. Kestler, D.P. et al. (1995) Blood 86:4559. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

参考图片

Detection of Human IL‑6 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with PHA. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑6 in Human PBMCs. IL‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND₅₀ is typically 15-45 ng/mL.