| 货号 | AF2049-SP |
| 别名 | BXMH1; CD307a antigen; CD307a; Fc receptor homolog 1; Fc receptor-like 1; FcRH1; FCRH1DKFZp667O1421; FcRL1; FcR-like protein 1; hIFGP1; IFGP family protein 1; IFGP1; IFGP1Fc receptor-like protein 1; Immune receptor translocation-associated protein 5; IRTA5; IRTA5immunoglobulin superfamily Fc receptor, gp42 | 全称 | Fc Receptor-like 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human FCRL1/FcRH1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ITRA4 is observed, and less than 5% cross‑reactivity with rhIRTA3, rhIRTA2, and rhIRTA1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 115350 (Human); 229499 (Mouse); 680665 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human FCRL1/FcRH1 Ala17-His304 Accession # Q96LA6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Fc Receptor-Like 1 (FCRL1), also known as FcRH1, and IRTA5, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified human IgG. Mature human FCRL1 consists of a 291 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 101 aa cytoplasmic domain with two immunotyrosine activation motifs (ITIMs) (3, 4). A charged glutamic acid within the transmembrane segment may mediate association with other signaling proteins. Alternative splicing may generate an isoform that lacks the transmembrane segment and an isoform that largely consists of the first two Ig-like domains (5). Mouse FCRL1 contains only two Ig-like domains, but it shares 62% aa sequence identity with homologous regions of the human FCRL1 ECD. FCRL1 is expressed on pre-B cells and naive B cells (6, 7). It is down‑regulated upon B cell activation but up‑regulated on memory B cells (6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (9‑11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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