Human IL-3 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-3 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-203-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-203-SP
别名	Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor
全称	Interleukin 3
反应种属	Human
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects human IL‑3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-3 and recombinant rat IL-3 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑3.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3562 (Human); 16187 (Mouse); 24495 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD123 targeting oncolytic adenoviruses suppress acute myeloid leukemia cell proliferation in vitro and in vivo. Authors: Li, G, Li, X, Wu, H, Yang, X, Zhang, Y, Chen, L, Wu, X, Cui, L, Wu, L, Luo, J, Liu, X Y Blood Cancer J, 2014;4(0):e194. Species: Human Sample Type: Cell Lysates Application: WB Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid. Authors: Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA Blood, 2008;112(9):3762-71. Species: Human Sample Type: Whole Cells Application: Neut Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions. Authors: Bartemes KR, Cooper KM, Drain KL, Kita H J. Allergy Clin. Immunol., 2005;116(4):827-35. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-3 Ala20-Phe152 Accession # Q6GS87
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells. IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Common beta Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Megakaryocytes T Cell Cytokine Signaling

参考图片

Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 Antibody. Recombinant Human IL‑3 (Catalog # 203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-203-NA). The ND₅₀ is typically 0.05‑0.25 µg/mL.