Human IL-1 alpha/IL-1F1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-1 alpha/IL-1F1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-200-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-200-SP
别名	Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
全称	Interleukin 1 alpha
反应种属	Human
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects human IL-1 alpha /IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) IL-1 alpha is observed and less than 5% cross-reactivity with recombinant porcine IL-1 alpha, recombinant rat IL‑1 alpha, recombinant cotton rat IL‑1 alpha, recombinant human IL-1 beta and rmIL-1 beta is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 4-20 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He Autophagy, 2017;0(0):0. Species: Human Sample Type: Whole Cells Application: Neut Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients PLoS ONE, 2016;11(9):e0162908. Species: Human Sample Type: Whole Cells Application: Bioassay P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC Br. J. Pharmacol., 2007;151(1):115-27. Species: Human Sample Type: Cell Culture Supernates Application: WB The response of human epithelial cells to TNF involves an inducible autocrine cascade. Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK Cell, 2006;124(6):1225-39. Species: Human Sample Type: Whole Cells Application: Neut Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1). Authors: Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C Cytokine, 2005;31(2):153-60. Species: Human Sample Type: Whole Cells Application: ICC Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha. Authors: Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF Circ. Res., 2003;93(6):515-22. Species: Human Sample Type: Whole Cells Application: ICC Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey. Authors: Sengupta J, Dhawan L, Ghosh D Cytokine, 2003;24(6):277-85. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-1 alpha /IL-1F1 Ser113-Ala271 Accession # Q53QF9
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia). IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis IL-1 Family Megakaryocytes Neuroinflammation Regulatory T Cells (Tregs) T Cell Cytokine Signaling

参考图片

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Human IL‑1 alpha /IL‑1F1 Antibody.

Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND₅₀ is typically 4-20 ng/mL in the presence of concanavalin A (1.25 µg/mL).

IL‑1 alpha /IL‑1F1 in Human PBMCs.

IL‑1 alpha /IL‑1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS using Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.