Human ERK1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human ERK1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1879-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1879-SP
别名	EC 2.7.11; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24
全称	Extracellular Signal-regulated Kinase 1
反应种属	Human
应用	Western Blot(0.2 µg/mL)
目标/特异性	Detects human ERK1 in Western blots. Does not detect recombinant or endogenous human ERK2 in Western blots.
使用方法	Western Blot: 0.2 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5595 (Human); 26417 (Mouse); 50689 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human ERK1 Ala2-Pro379 Accession # P27361
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ERK1 (also known as MAPK3, Mitogen-activated protein kinase 3) is a 44 kDa phosphoprotein. It is Serine/threonine proteinkinase which acts as an essential component of the MAP kinase signal transduction pathway. ERK1 was initially isolated and cloned as a kinase activated in response to insulin and NGF. It is expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates human ERK1 at Thr202/Tyr204. Within the range used as an immunogen human and mouse ERK1 share 97% amino acid sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases Cancer Biomarkers Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family C-type Lectin Receptors EMT Induction GDNF Family IL-1 Family IL-17 Family Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Mini-review: The ERK Signal Transduction Pathway Natural Killer T (NKT) Cells Neurotrophin/Trk Family PDGF Family Positive Regulators of the Jak/STAT Pathway ROS and Stress Resistance TNF Superfamily Regulators VEGF Family

参考图片

Detection of Human ERK1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1879) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.