Human/Mouse/Rat JNK2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat JNK2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1846-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1846-SP
别名	c-Jun kinase 2; c-Jun N-terminal kinase 2; EC 2.7.11; EC 2.7.11.24; JNK2A; JNK2BETA; JNK2JNK2ALPHA; JNK-55; Jun kinase; MAP kinase 9; MAPK 9; MAPK9; mitogen-activated protein kinase 9; p54a; p54aSAPK; PRKM9JNK2B; SAPK; SAPK1 alpha; Stress-activated protein kinase JNK2
全称	C-Jun N-terminal Kinase 2
反应种属	Human/Mouse/Rat
应用	Western Blot(0.2 µg/mL)
目标/特异性	Detects human, mouse, and rat JNK2 in Western blots. Recognizes p54 JNK (isoforms 1 and/or 4, both 424 aa) and p46 JNK (isoforms 2 and/or 3, both 382 aa) in Western blots. Because the peptide immunogen corresponds to a region of JNK2 with low homology to other JNKs, the antibody does not detect recombinant JNK1 or JNK3.
使用方法	Western Blot: 0.2 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5601 (Human); 243911 (Mouse); 50658 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Human, Mouse, and Rat JNK2 synthetic peptide PGIKFEELFPDWIFPSESERD
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Members of the MAPK family, the c-Jun N-terminal kinases (JNKs) are activated by environmental stresses and inflammatory cytokines. Ten JNK isoforms are created by alternative splicing of mRNA transcripts derived from three genes: JNK1, JNK2, and JNK3. All JNKs are activated by dual phosphorylation; at T183/Y185 for JNK1 and 2, and T221/Y223 for JNK3. Activated JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases C-type Lectin Receptors GDNF Family IL-1 Family Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family NOD-like Receptors and the Inflammasome TNF Superfamily Regulators Toll-Like Receptors Wnt Intracellular Signaling

参考图片

Detection of Human and Mouse JNK2 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). specific bands for JNK2 was detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of JNK2 by Western Blot. Western blot shows recombinant JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for JNK2 was detected at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.