Human Islet-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Islet-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1837-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1837-SP
别名	insulin gene enhancer protein ISL-1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain, (islet-1); ISL1; Isl-1; ISLET1; islet-1
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3670 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells Sci Rep, 2016;6(0):25474. Species: Human Sample Type: Whole Cells Application: IHC - Paraffin embedded Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Authors: Adams, Katrina, Rousso, David L, Umbach, Joy A, Novitch, Bennett Nat Commun, 2015;6(0):6778. Species: Mouse Sample Type: Tissue Homogenates Application: IHC Not Specified LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice. Authors: Chen J, Fu R, Cui Y, Li Y, Pan J, Liu J, Luo H, Yin J, Li D, Cui S J Biol Chem, 2013;288(17):12395-405. Species: Mouse Sample Type: Cell Lysates Application: WB Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord. Authors: Hazen VM, Phan KD, Hudiburgh S, Butler SJ Dev. Biol., 2011;356(2):566-75. Species: Chicken Sample Type: Whole Tissue Application: IHC Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells. Authors: Yau WW, Tang MK, Chen E Proteome Sci, 2011;9(1):3. Species: Mouse Sample Type: Whole Cells Application: ICC Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Authors: Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE Stem Cells, 2009;27(4):806-11. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Islet-1 Met4-Ala349 Accession # P61371
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Islet-1 is a member of a family of homeodomain containing transcriptionfactors. It is expressed in all islet cells in the pancreas and is anearly marker for motor neuron differentiation.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cardiac Stem Cell Markers Homeodomain (Hox) Transcription Factors ICC / IHC Images: Islet-1 Motor Neuronal Markers Pancreatic Endoderm Pancreatic Endoderm Cell Markers Transcription Factors and Regulators

参考图片

Islet‑1 in iPS2 Human Stem Cells. Islet‑1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.