| 货号 | AF1828-SP |
| 别名 | Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2 | 全称 | Triggering Receptor Expressed on Myeloid Cells 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TREM-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TREM-2b is observed and less than 1% cross-reactivity with recombinant human (rh) TREM-1, rhTLT-1, rmTREM-L2, rmTREM-3 and rmTREM-4 is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 54209 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TREM-2 His19-Ser174 Accession # Q9NZC2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TREM-2 is a single Ig domain receptor expressed by cells of myeloidlineage. It is a pattern recognition receptor that binds anionicligands. TREM-2 associates with the adapter protein DAP12 to deliver anactivating signal that plays a role in both innate and adaptive immuneresponses. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TREM‑2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TREM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM‑2 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
TREM‑2 in Human Dendritic Cells. TREM‑2 was detected in immersion fixed immature human dendritic cells using Goat Anti-Human TREM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
