| 货号 | AF1827-SP |
| 别名 | DDC-4; Frizzled protein, human endometrium; FRP-4; FRP-AP; frpHE; FRPHEMGC26498; FrzB2; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; sFRP-4 | 全称 | Secreted Frizzled Related Protein 4 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human sFRP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) sFRP-4 is observed, and less than 5% cross-reactivity with recombinant human (rh) sFRP-3, rhsFRP-1, and rmsFRP-2 is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6424 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human sFRP-4 Ala22-Val346 Accession # AAC04617 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | sFRP-4, also known as DDC-4, Frp, FRPHE and Secreted Frizzled Related Protein 4, is a 50-55 kDa glycoprotein expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human sFRP‑4 by Western Blot. Western blot shows lysates of OVCAR‑3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human sFRP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for sFRP‑4 at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
