| 货号 | AF1810-SP |
| 别名 | endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Endocan in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Endocan is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 20 µg/mL Recombinant Human Endocan/ESM‑1. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11082 (Human); 71690 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Upregulation of endocan by Epstein-Barr virus latent membrane protein 1 and its clinical significance in nasopharyngeal carcinoma. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Endocan/ESM-1 Trp20-Arg184 Accession # Q9NQ30 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Endocan, also known as endothelial-cell specific molecule-1 (ESM-1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung (1). Endocan expression has also been detected in different epithelia and in adipocytes (2, 3). The expression of endocan is upregulated by TNF‑ alpha, IL-1 beta, or lipopolysaccharide and down-regulated by IFN‑ gamma (1). The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues (1). The DS chain is covalently attached to serine 137 (4). Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen-1, LFA-1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA-1-mediated leukocyte activation (5). Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity (3, 6). Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo (3, 6). Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported (2, 6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Endocan/ESM‑1 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endocan/ESM‑1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Cell Adhesion Mediated by Endocan/ESM‑1 and Neutralization by Human Endocan/ESM‑1 Antibody. Recombinant Human Endocan/ESM‑1 (Catalog # 1810‑EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM‑1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810). The ND50 is typically 1-4 µg/mL. |
