| 货号 | AF1808-SP |
| 别名 | CHL; chordin-like 1; chordin-like protein 1; CHRDL1; CRDL1; dA141H5.1; Neuralin 1; neuralin-1; neurogenesin-1; NRLN1; NRLN1neuralin 1; Ventroptin alpha; ventroptin; VOPT |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Chordin-like 1/CHRDL1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 91851 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Chordin-like 1/CHRDL1 Glu28-Cys457 Accession # NP_001137454 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Chordin-like 1 (CHRDL1) also known as Chordin-like, ventroptin, and neuralin, is a secreted glycoprotein that has significant homology to chordin and acts as a bone morphogenetic protein (BMP) antagonist (1‑3). Human Chordin-Like 1 cDNA encodes a 450 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Chordin-Like 1 contains three internal cysteine-rich procollagen repeats (CRs) that are conserved in the spacing of their ten cysteines. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding (1). The CR1 and CR3 of Chordin-Like 1 are most similar to CR3 of chordin (1). Chordin-Like 1 and chordin exhibit similar BMP inhibitory activity in vivo and directly interact with BMP-4 in vitro (1, 2). However, Chordin-Like 1 differs in its spatial and temporal expression from chordin. Mouse Chordin-Like 1 is detected in mesenchyme-derived cells such as the dermatome and limb bud, as well as chondrocytes of the developing skeleton later in development. In addition, Chordin-Like 1 is expressed embryonically in the gastrointestinal tract, kidney tubules, neural tube, CNS, cerebellum, derivatives of the neural crest cells, developing hair follicles, and the olfactory bulb where it is expressed in a gradient in the retina (1‑3). Chordin-Like 1 is also expressed in adult marrow stromal cells. Human CHRDL1 and the long form of mouse CHRDL1 share 93% aa sequence identity. Multiple forms of human Chordin-Like 1 containing small deletions or insertions (including an E insertion between P94 and D95, a GKKAK deletion (aa residues 318‑322), and/or another E insertion between K322 and E323) have been described. Similar isoforms have also been reported for mouse Chordin-Like 1. In addition, a short form of mouse Chordin-Like 1 with a shortened variant C-terminus but with all 3 CR domains intact also exists (1). These multiple forms were attributed to alternative splicing and transcriptional termination sites within the coding region of Chordin-Like 1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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