| 货号 | AF17991-SP |
| 别名 | Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3 | 全称 | Signal Transducer and Activator of Transcription 3 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human and mouse STAT3 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6774 (Human); 20848 (Mouse); 25125 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cyclophilins contribute to Stat3 signaling and survival of multiple myeloma cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human STAT3 Met1-Asn175 Accession # P40763 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
STAT3 in Human PBMCs. STAT3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Sheep Anti-Human STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17991) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
Detection of Human STAT3 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, Raji human Burkitts lymphoma cell line, and Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17991) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STAT3 at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
