Human Cadherin-11 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Cadherin-11 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1790-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1790-SP
别名	CAD11OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); cadherin-11; CDH11; CDHOB; OB; OB-Cadherin; Osteoblast cadherin
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Cadherin-11 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Cadherin‑11 is observed, and approximately 10% cross-reactivity with recombinant human (rh) Cadherin‑12 is observed, and less than 5% cross-reactivity with rhK-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhE‑Cadherin, rhN‑Cadherin, rhP‑Cadherin, rhVE‑Cadherin, rhCadherin-13, and rhCadherin‑17 is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Adhesion Blockade: The adhesion of MDA-MB-231 human breast cancer cells (5 x 10⁴ cells/well) to immobilized Recombinant Human Cadherin-11 Fc Chimera (Catalog # 1790-CA, 20 µg/mL, 100 µL/well) was maximally inhibited (70-100%) by 30 µg/mL of the antibody.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1009 (Human); 12552 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. N-cadherin-mediated cell-cell adhesion promotes cell migration in a three-dimensional matrix. Authors: Shih W, Yamada S J Cell Sci, 2012;125(0):3661-70. Species: Canine Sample Type: Cell Lysates Application: WB Cadherin-11 promotes the metastasis of prostate cancer cells to bone. Authors: Chu K, Cheng CJ, Ye X, Lee YC, Zurita AJ, Chen DT, Yu-Lee LY, Zhang S, Yeh ET, Hu MC, Logothetis CJ, Lin SH Mol. Cancer Res., 2008;6(8):1259-67. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking. Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD Biotechnol. Bioeng., 2007;97(6):1617-25. Species: Human Sample Type: Cell Lysates Application: WB E-cadherin cell-cell adhesion in ewing tumor cells mediates suppression of anoikis through activation of the ErbB4 tyrosine kinase. Authors: Kang HG, Jenabi JM, Zhang J, Keshelava N, Shimada H, May WA, Ng T, Reynolds CP, Triche TJ, Sorensen PH Cancer Res., 2007;67(7):3094-105. Species: Human Sample Type: Whole Cells Application: AdBlk Secretion and organization of a cornea-like tissue in vitro by stem cells from human corneal stroma. Authors: Du Y, Sundarraj N, Funderburgh ML, Harvey SA, Birk DE, Funderburgh JL Invest. Ophthalmol. Vis. Sci., 2007;48(11):5038-45. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin‑11 Phe23-Thr617 Accession # AAA35622
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The cadherin superfamily comprises a large number of membrane glycoproteins with one or more cadherin repeats, which are involved in Ca²⁺ dependent cell-cell adhesion. The family can be subdivided into several major subgroups, including the type I and type II classical cadherins, desmosomal cadherins, protocadherins, seven transmembrane (Flamingo) cadherins, FAT-family cadherins, T-cadherin and other unclassified cadherins (1). Cadherin-11, also known as OB-cadherin, is a type II classical cadherin. Classical cadherins are type I transmembrane proteins with an N-terminal extracellular domain containing five tandem cadherin repeats and a C-terminal cytoplasmic domain with a characteristic sequence for binding to catenins. Type I cadherins (E-, N-, P-, R-, M-, and EP-cadherin) differ from type II cadherins (cadherin-5 to -12, -18 to -20 and -22) by the presence of the HAV tripeptide motif in the most N-terminal cadherin repeat (2). Classic cadherins mediate cell-cell adhesion preferentially via homotypic interactions and form adherens juctions that have beta -catenin and p120 (ctn) at the cytoplasmic side of the junction (3, 4). Homotypic cadherin interactions also transduce outside-in and inside-out cell signals. Cadherin signaling induces various cellular processes including cell motility, actin cytoskeleton reorganization, proliferation, and differentiation (3, 4). Cadherin-11 is expressed in a variety or normal tissues of mesodermal origin including areas of the kidney and brain, in normal osteoblasts, and in tumors of the stomach, kidney, colon, breast, and bone (osteosarcoma) (5, 6). It is also differentially expressed in the embryonic brain and may be important in regulating neural development. Human Cadherin-11 exhibits a unique mRNA splice site allowing for two forms of the protein to be expressed, a full-length 796 amino acid (aa) protein and a COOH terminus-truncated variant of 693 aa. The truncated variant has a unique cytoplasmic region due to a frameshift event (3). The full-length human and mouse Cadherin-11 share 97% homology at the aa sequence level.
运输条件	Blue Ice
存放说明	4℃
参考文献	Angst, B.D. et al. (2001) J. Cell Sci. 113:629. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136. Feltes, C.M. et al. (2002) Cancer Research. 62:6688. Wheelock, J.J. and K.R. Johnson (2003) Annu. Rev. Cell Dev. Biol. 19:207. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337. Pishvaian, M.J. et. al. (1999) Cancer Research 59:947.

参考图片

Detection of Human Cadherin‑11 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cadherin‑11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cadherin‑11 in Human Prostate Cancer Tissue. Cadherin‑11 was detected in fomralin fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Cadherin‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized in the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.