Human IL-17 RA/IL-17 R Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-17 RA/IL-17 R Affinity Purified Polyclonal Ab (25 UG)

货号： AF177-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF177-SP
别名	CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 receptor A; IL17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
全称	Interleukin 17 Receptor
反应种属	Human
应用	Western Blot(0.5 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL‑17 RA/IL‑17 R is observed.
使用方法	Western Blot: 0.5 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in normal human dermal fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND₅₀) is typically 0.1-0.5 µg/mL in the presence of 25 ng/mL Recombinant Human IL‑17.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	23765 (Human); 16172 (Mouse); 312679 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients. Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren PLoS ONE, 2014;9(9):e106834. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma. Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D Int. J. Cancer, 2009;125(5):1092-101. Species: Human Sample Type: Whole Cells Application: Flow Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL J. Immunol., 2008;181(6):4299-310. Species: Human Sample Type: Whole Cells Application: Neut The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. Authors: Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM J. Immunol., 2008;181(4):2799-805. Species: Human Sample Type: Cell Lysates Application: WB IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R J. Immunol., 2004;173(5):3482-91. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R Leu33-Trp320 Accession # Q96F46
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL-17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
运输条件	Blue Ice
存放说明	4℃
参考文献	Yao, Z. et al. (1997) Cytokine 9:794. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

参考图片

Detection of Human IL‑17 RA/IL‑17 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑17 RA/IL‑17 R at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑6 secretion Induced by IL‑17 and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in normal human dermal fibroblasts in a dose-dependent manner (orange line), as measured by the Human IL‑6 Quantikine ELISA Kit (Catalog # D6050). IL‑6 secretion elicited by Recombinant Human IL‑17 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177). The ND₅₀ is typically 0.1-0.5 µg/mL.