| 货号 | AF1779-SP |
| 别名 | Eryf1; ERYF1GATA-binding protein 1 (globin transcription factor 1); GATA binding protein 1 (globin transcription factor 1); GATA-1erythroid transcription factor; GATA-binding factor 1; GF-1; GF1globin transcription factor 1; NF-E1 DNA-binding protein; NFE1erythroid transcription factor 1; transcription factor GATA1; XLTT |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human GATA-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with rhGATA-2, rhGATA-5 and rhGATA-6. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2623 (Human); 14460 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GATA-1 Met1-Ser413 Accession # P15976 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA‑1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human GATA‑1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL of Human GATA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1779) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-1 at approximately 50 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
