Human/Mouse/Rat Akt1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat Akt1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF17751-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF17751-SP
别名	AKT; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKBMGC99656; PRKBA; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; v-akt murine thymoma viral oncogene homolog 1
全称	v-Akt Murine Thymoma Viral Oncogene Homolog 1
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human, mouse, and rat Akt1 in Western blots. The antibody is specific for Akt1 and does not detect recombinant Akt2 or Akt3.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	207 (Human); 11651 (Mouse); 25233 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Akt1 Met1-Glu149 Accession # P31749
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.
运输条件	Blue Ice
存放说明	4℃
参考文献	A Novel Pathway for TOR-independent Autophagy Regulation by Akt Akt Modulates TGF-beta Activities Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family Diabetic Peripheral Neuropathy GDNF Family Growth Hormone/IGF-I Axis Human Phospho-MAPK Array Kit ICC / IHC Images: Akt1 Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family Notch Pathway PDGF Family TNF Superfamily Regulators VEGF Family

参考图片

Detection of Human/Mouse/Rat Akt1 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and HL-60 human promyelocytic leukemia cell line, and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Akt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17751) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775‑KS), recombinant human Akt2, and recombinant human Akt3 (2 ng/lane) were included. A specific band for Akt1 was detected at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.