Human Oct-3/4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Oct-3/4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1759-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1759-SP
别名	Otf3g; Otf4; Pou5f1
全称	Octamer-binding Protein 3/4
反应种属	Human
应用	Western Blot,Simple Western,Immunoprecipitation,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry
目标/特异性	Detects human Oct-3/4 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 20 µg/mL Immunoprecipitation: 3 µg/10⁶cells Chromatin Immunoprecipitation (ChIP): 5 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5460 (Human); 18999 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells. Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen Sci Rep, 2014;4(0):3779. Species: Human Sample Type: Cell Lysates Application: WB Pluripotent stem cells derived from mouse and human white mature adipocytes. Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K Stem Cells Transl Med, 2014;3(2):161-71. Species: Mouse Sample Type: Whole Cells Application: ICC Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji PLoS ONE, 2012;7(9):e45435. Species: Human Sample Type: Whole Cells Application: ICC Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties. Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL FASEB J., 2011;25(0):2604-14. Species: Rabbit Sample Type: Whole Cells Application: ICC A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells. Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ Stem Cells, 2011;29(10):1517-27. Species: Human Sample Type: Cell Lysates Application: ChIP SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells. Authors: Chng Z, Teo A, Pedersen RA, Vallier L Cell Stem Cell, 2010;6(1):59-70. Species: Human Sample Type: Cell Lysates Application: ChIP Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Authors: Fong H, Hohenstein KA, Donovan PJ Stem Cells, 2008;26(8):1931-8. Species: Human Sample Type: Whole Cells Application: ICC Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Stem Cells, 2007;25(2):490-9. Species: Human Sample Type: Whole Cells Application: Flow A comparison of NIH-approved human ESC lines. Authors: Ware CB, Nelson AM, Blau CA Stem Cells, 2006;24(12):2677-84. Species: Human Sample Type: Whole Cells Application: ICC Controlled-rate freezing of human ES cells. Authors: Ware CB, Nelson AM, Blau CA BioTechniques, 2005;38(6):879-80, 882-3. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) Accession # Q01860
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Scholer, H.R. et al. (1990) Nature 344:435. Scholer, H.R. et al. (1989) EMBO J.8:2543. Rosner, M.H. et al. (1990) Nature345:686. Niwa, H. et al. (2000) Nat. Genet. 24:372. Pesce, M. et al. (2001) Stem Cells 19:271.

参考图片

Detection of Human and Mouse Oct‑3/4 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Oct‑3/4 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Oct‑3/4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.

Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline phosphatase (ALPL) and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Oct‑3/4 by Simple Western^TM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.