Human TACI/TNFRSF13B Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TACI/TNFRSF13B Affinity Purified Polyclonal Ab (25 UG)

货号： AF174-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF174-SP
别名	CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B
全称	Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human TACI in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TACI and recombinant human BCMA is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.075-0.3 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	23495 (Human); 57916 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F Leukemia, 2016;30(1):163-72. Species: Human Sample Type: Whole Cells Application: Neut The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity. Authors: Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E J Immunol, 2015;194(2):542-52. Species: Human Sample Type: cell culture supernate Application: IP TACI mutations and impaired B-cell function in subjects with CVID and healthy heterozygotes. Authors: Martinez-Gallo M, Radigan L, Almejun M, Martinez-Pomar N, Matamoros N, Cunningham-Rundles C J Allergy Clin Immunol, 2013;131(2):468-76. Species: Human Sample Type: whole cells Application: Flow The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88. Authors: He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, Shan M, Xiong H, Bussel JB, Chiu A, Puel A, Reichenbach J, Marodi L, Doffinger R, Vasconcelos J, Issekutz A, Krause J, Davies G, Li X, Grimbacher B, Plebani A, Meffre E, Picard C, Cunningham-Rundles C, Casanova JL, Cerutti A Nat. Immunol., 2010;11(9):836-45. Species: Human Sample Type: Cell Lysates Application: WB Identification of transitional type II B cells in the salivary glands of patients with Sjogren's syndrome. Authors: Daridon C, Pers JO, Devauchelle V, Martins-Carvalho C, Hutin P, Pennec YL, Saraux A, Youinou P Arthritis Rheum., 2006;54(7):2280-8. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B Ser2-Thr166 Accession # Q53F36
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Xia, X-Z.et al. (2000) J. Exp. Med. 192:137. von Bulow, G.U.et al. (1997) Science 278:138. Gross, J.A.et al. (2000) Nature 404:995. Marsters, S.A.et al. (2000) Curr. Biol. 10:785. Yan, M.et al. (2000) Nature Immunol. 1:37. Wu, Y.et al. (2000) J. Biol. Chem. 275:35478.

参考图片

TACI/TNFRSF13B in Human Spleen.

TACI/TNFRSF13B was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human TACI/TNFRSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF174) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to lymphocytes in the marginal zone. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.