| 货号 | AF1700-SP |
| 别名 | GATA binding protein 6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human GATA-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human GATA-5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2627 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Inhibition of transforming growth factor beta (TGF-beta) signaling can substitute for Oct4 protein in reprogramming and maintain pluripotency. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GATA-6 Met1-Thr449 Accession # Q92908 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GATA-6 (also named GATA-GT1) is a zinc finger transcription activatingprotein that bins to a T/A-G-A-T-A-G/A DNA sequence motif inmesodermally and endodermally-derived tissue. There are two GATA-6isoforms that arise from alternate start sites: a short form of 449amino acids (aa), and a long form of 595 aa. The short form of humanGATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat andmouse, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of GATA‑6-regulated Genes by Chromatin Immunoprecipitation. KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR. | |
GATA‑6 in KATO-III Human Cell Line. GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
GATA‑6 in Human Intestine. GATA‑6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
