Human ICOS Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human ICOS Affinity Purified Polyclonal Ab (25 UG)

货号： AF169-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF169-SP
别名	Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850
全称	Inducible Co-stimulator
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human ICOS in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse ICOS is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	29851 (Human); 54167 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human ICOS Glu21-Phe141 Accession # Q9Y6W8
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_hdifferentiation.
运输条件	Blue Ice
存放说明	4℃
参考文献	Aicher, A. et al. (2000) J. Immunol. 164:4689. Coyle, A.J. et al. (2000) Immunity 13:95. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1. Hutloff, A. et al. (1999) Nature 397:263. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. Yoshinaga, S.K. et al. (1999) Nature 402:827.

参考图片

ICOS in Human Tonsil. ICOS was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human ICOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.