Human Kallikrein 11 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Kallikrein 11 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1595-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1595-SP
别名	EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Hippostasin; hK11; kallikrein 11; kallikrein-related peptidase 11; KLK11; MGC33060; PRSS20kallikrein-11; Serine protease 20; TLSPhippostasin; Trypsin-like protease
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human Kallikrein 11 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 5, and rhKallikrein 9 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	11012 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Enzymatic properties of human kallikrein-related peptidase 12 (KLK12). Authors: Memari</LastName><ForeNam N</Initial, Memari N, Jiang W, Diamandis EP, Luo LY Biol. Chem., 2007;388(4):427-35. Species: Human Sample Type: recombinant KLK11 Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11 Ile54-Asn282 Accession # Q9UBX7
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.
运输条件	Blue Ice
存放说明	4℃
参考文献	Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184. Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225. Yousef, G.M. et al. (2000) Genomics 63:88. Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205. Gan, L. et al. (2000) Gene 257:119. Diamandis, E.P. et al. (2002) Cancer Res. 62:295. Nakamura, T. et al. (2003) Urology 61:1042. Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.

参考图片

Kallikrein 11 in Human Prostate. Kallikrein 11 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Kallikrein 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1595) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.