| 货号 | AF1573-SP |
| 别名 | ATV; AT-V1; AT-V2; Cell cycle regulatory protein p95; FLJ10155; MGC87362; NBN; NBS1P95; NBSNijmegen breakage syndrome protein 1; Nibrin; Nijmegen breakage syndrome 1 (nibrin); p95 protein of the MRE11/RAD50 complex; p95 | 全称 | Nijmegen Breakage Syndrome 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunoprecipitation(2 µg/500 µg cell lysate) |
| 目标/特异性 | Detects human Nbs1 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunoprecipitation: 2 µg/500 µg cell lysate |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4683 (Human); 27354 (Mouse); 85482 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Nbs1 Pro498-Arg754 Accession # O60934 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TheNijmegenBreakageSyndrome1 (Nbs1) protein is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. In fact, mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Nbs1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nbs1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
