| 货号 | AF1560-SP |
| 别名 | FALLOTEIN; PDGF-C; platelet derived growth factor C; platelet-derived growth factor C; SCDGFSpinal cord-derived growth factor; secretory growth factor-like protein; VEGF-E | 全称 | Platelet-derived Growth Factor C |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human PDGF-C in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse PDGF-C is observed, and less than 1% cross-reactivity with human PDGF, recombinant human (rh) PDGF‑AA, rhPDGF‑BB, rhPDGF‑AB, and rhPDGF‑D is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize PDGF‑CC-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 0.8 µg/mL Recombinant Human PDGF‑CC. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56034 (Human); 54635 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PDGF-C Gly230-Gly345 Accession # Q9NRA1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Platelet-derived growth factor C (PDGF-C), also named spinal cord derived growth factor (SCDGF) andfollotain, is a member of the PDGF family that binds to the PDGFreceptor alpha alpha and alpha beta. PDGF-C is a growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It is a potent mitogen and chemoattractant for cells of mesenchymal origin. It is also required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. In addition, PDGF-C is required for normal skin morphogenesis during embryonic development. PDGF-C plays an important role in angiogenesis and blood vessel development and is involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintainance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Western blot analysis of platelets identified 55 kDa and 80 kDa PDGF C forms that were secreted on platelet activation (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | Fang L. et al. (2004) Arterioscler Thromb Vasc Biol. 24:787 |
Detection of Human PDGF‑C by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PDGF‑C p80 at approximately 80 kDa and PDGF‑C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
PDGF‑C in Human Pancreatic Cancer Tissue. PDGF‑C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 µg/mL Goat Anti-Human PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Cell Proliferation Induced by PDGF‑CC and Neutralization by Human PDGF‑C Antibody. Recombinant Human PDGF‑CC (Catalog # 1687-CC) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560). The ND50 is typically 6-24 µg/mL. |
