Human Axl Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Axl Affinity Purified Polyclonal Ab (25 UG)

货号： AF154-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF154-SP
别名	Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL
全称	Axl Receptor Tyrosine Kinase
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 3-10 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human Gas6, lacking the Gla domain, to immobilized Recombinant Human Axl Fc Chimera (Catalog # 154-AL) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	558 (Human); 26362 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers J Clin Invest, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Clinical, Pathological, and Molecular Features of Lung Adenocarcinomas with AXL Expression Authors: K Sato, K Suda, S Shimizu, K Sakai, H Mizuuchi, K Tomizawa, T Takemoto, K Nishio, T Mitsudomi PLoS ONE, 2016;11(4):e0154186. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The Axl-Regulating Tumor Suppressor miR-34a Is Increased in ccRCC but Does Not Correlate with Axl mRNA or Axl Protein Levels. Authors: Fritz H, Gustafsson A, Ljungberg B, Ceder Y, Axelson H, Dahlback B PLoS ONE, 2015;10(8):e0135991. Species: Human Sample Type: Whole Cells Application: Flow MET and AXL inhibitor NPS-1034 exerts efficacy against lung cancer cells resistant to EGFR kinase inhibitors because of MET or AXL activation. Authors: Rho, Jin Kyun, Choi, Yun Jung, Kim, Seon Ye, Kim, Tae Won, Choi, Eun Kyun, Yoon, Seon-Joo, Park, Bu Man, Park, Eunhye, Bae, Jong Hwa, Choi, Chang-Mi, Lee, Jae Cheo Cancer Res, 2014;74(1):253-62. Species: Human Sample Type: Cell Lysate Application: WB Mechanisms of acquired resistance to EGFR-tyrosine kinase inhibitor in Korean patients with lung cancer. Authors: Ji, Wonjun, Choi, Chang-Mi, Rho, Jin Kyun, Jang, Se Jin, Park, Young So, Chun, Sung-Min, Kim, Woo Sung, Lee, Jung-Shi, Kim, Sang-We, Lee, Dae Ho, Lee, Jae Cheo BMC Cancer, 2013;13(0):606. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Authors: Zhang Z, Lee JC Nat. Genet., 2012;44(8):852-860. Species: Human Sample Type: Cell Lysates Application: IP Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells. Authors: Brown JE, Krodel M, Pazos M PLoS ONE, 2012;7(5):e36800. Species: Human Sample Type: Cell Lysates Application: IP Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y J. Virol., 2012;86(4):2067-78. Species: Human Sample Type: Whole Cells Application: Flow Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. Species: Human Sample Type: Whole Cells Application: Neut Prolonged exposure to a Mer ligand in leukemia: Gas6 favors expression of a partial Mer glycoform and reveals a novel role for Mer in the nucleus. Authors: Migdall-Wilson J, Bates C, Schlegel J, Brandao L, Linger RM, Deryckere D, Graham DK PLoS ONE, 2012;7(2):e31635. Species: Human Sample Type: Cell Lysates Application: WB Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Thromb. Haemost., 2011;107(1):140-9. Species: Human Sample Type: Whole Cells Application: ICC Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Authors: Butler GS, Dean RA, Tam EM, Overall CM Mol. Cell. Biol., 2008;28(15):4896-914. Species: Human Sample Type: Cell Lysates Application: WB Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. Authors: Uehara H, Shacter E J. Immunol., 2008;180(4):2522-30. Species: Human Sample Type: Cell Lysates Application: WB Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells. Authors: Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP, Canfield AE Circ. Res., 2007;100(4):502-9. Species: Bovine Sample Type: Cell Lysates Application: IP Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA J. Thromb. Haemost., 2005;3(4):733-41. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Axl Glu33-Pro440 Accession # AAA61243
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.
运输条件	Blue Ice
存放说明	4℃
参考文献	Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022. Holland, S. et al. (2005) Canc. Res. 65:9294.

参考图片

Detection of Human Axl by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Axl in Human Stomach. Axl was detected in immersion fixed paraffin-embedded sections of human stomach using 10 µg/mL Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to endothelial cells in the vasculature of the longitudinal muscle layer. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.