Human IL-13 R alpha 1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-13 R alpha 1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF152-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF152-SP
别名	bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13RA1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4
全称	Interleukin 13 Receptor alpha 1
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human IL‑13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinat human (rh) IL-13 R alpha 2, recombinant mouse IL-13 R alpha 1, rhIL-5 R alpha, rhIL-5 R beta, rhIL-4 R, and rhIL-9 R is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3597 (Human); 16164 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells. Authors: Reece P, Gauvreau G, Sehmi R, Denburg J PLoS ONE, 2014;9(6):e100734. Species: Human Sample Type: Whole Cells Application: Neut Obligatory role for interleukin-13 in obstructive lesion development in airway allografts. Authors: Lama VN, Harada H, Badri LN, Flint A, Hogaboam CM, McKenzie A, Martinez FJ, Toews GB, Moore BB, Pinsky DJ Am. J. Pathol., 2006;169(1):47-60. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis. Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M Int. J. Oncol., 2004;25(3):605-13. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Differential effect of IL-4 and IL-13 on the expression of recombination-activating genes in mature B cells from human peripheral blood. Authors: Kajiwara K, Shinazawa M, Morishima H, Yanagihara Y Cell. Immunol., 2004;227(2):121-8. Species: Human Sample Type: Whole Cells Application: Flow Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Authors: Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ Am. J. Pathol., 2002;160(4):1405-23. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1 Ala27-Thr343 (Thr130Ile) Accession # Q5JSL4
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha , is now referred to as IL-13 R alpha 2. The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL‑13 R alpha 1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL‑13 R alpha 1 is also closely related to that of IL-13 R alpha 2. IL-13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.
运输条件	Blue Ice
存放说明	4℃
参考文献	Caput, D.et al. (1996) J. Biol. Chem. 271:16921. Donaldson, D.D.et al. (1998) J. Immunol. 161:2317. Aman, M.J.et al. (1996) J. Biol. Chem. 271:29265. Hilton, D.J.et al. (1996) Proc. Natl. Acad. Sci. USA 93:497. Zhang, J.G.et al. (1997) J. Biol. Chem. 272:9474.

参考图片

Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 R alpha 1 Antibody. Recombinant Human IL‑13 (Catalog # 213‑ILB) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND₅₀ is typically 10‑30 µg/mL.

Detection of IL‑13 R alpha 1 in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF152, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

IL‑13 R alpha 1 in Human Skin. IL‑13 R alpha 1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.