| 货号 | AF149-SP |
| 别名 | AD3EC 3.4.23.-; Alzheimer disease 3; EC 3.4.23; FAD; presenilin 1; Protein S182; PS-1; PS1presenilin-1; PSEN1; PSNL1; S182 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human PS-1 NTF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) PS-1 CTF (aa 298-407) and rhPS-2 NTF (aa 1-80) is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5663 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Erlin-2 is associated with active gamma-secretase in brain and affects amyloid beta-peptide production. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Presenilin-1 Lys1-Lys80 Accession # P49768 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Presenilin-1 (PS-1) and Presenilin-2 (PS-2) are integral membrane proteins that contain multiple transmembrane domains. Both are predominantly located within the endoplasmic reticulum (ER) and early Golgi apparatus. They are primarily expressed in neurons and are ubiquitously expressed in the brain. PS-1 and PS-2 are proteolytically cleaved to generate an N-terminal fragment (NTF) and a C-terminal fragment (CTF). Mutations in the gene for Presenilin may play an important role in the pathology of Alzheimers disease. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Presenilin‑1 in Human Brain. Presenilin‑1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 15 µg/mL Goat Anti-Human Presenilin‑1 N‑Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF149) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
