Human Decorin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Decorin Affinity Purified Polyclonal Ab (25 UG)

货号： AF143-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF143-SP
别名	Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B
反应种属	Human
应用	Western Blot(0.1 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(10-20 µg/mL)
目标/特异性	Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Decorin is observed.
使用方法	Western Blot: 0.1 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 10-20 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1634 (Human); 13179 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors. Authors: Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S J Biol Chem, 2015;290(12):7658-70. Species: Human Sample Type: Cell Lysates Application: WB Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Invest Ophthalmol Vis Sci, 2013;54(2):1014-25. Species: Human Sample Type: protein Application: WB Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO J. Biol. Chem., 2011;286(4):2636-47. Species: Human Sample Type: Cell Lysates Application: WB Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking. Authors: Zhang Y, Conrad AH, Conrad GW J. Biol. Chem., 2011;286(15):13011-22. Species: Bovine Sample Type: Tissue Homogenates Application: WB Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease. Authors: Ranjzad P, Salem HK, Kingston PA Gene Ther., 2009;16(9):1154-62. Species: Human Sample Type: Whole Tissue Application: IHC Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium. Authors: Kitaya K, Yasuo T J. Leukoc. Biol., 2009;85(3):391-400. Species: Human Sample Type: Tissue Homogenates Application: WB Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. Authors: Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R J. Pathol., 2009;218(1):131-42. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Decorin is a secreted protein associated with obesity and type 2 diabetes. Authors: Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K Int J Obes (Lond), 2008;32(7):1113-21. Species: Human Sample Type: Plasma Application: ELISA Development Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic. Authors: Lallam-Laroye C, Escartin Q, Zlowodzki AS, Barritault D, Caruelle JP, Baroukh B, Saffar JL, Colombier ML J Biomed Mater Res A, 2006;79(3):675-83. Species: Hamster Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin Gly17-Lys359 Accession # P07585
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.
运输条件	Blue Ice
存放说明	4℃
参考文献	Naito, Z. (2005) J. Nippon Med. Sch. 72:137. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771. Danielson, K. et al. (1993) Genomics 15:146.

参考图片

Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Decorin in Human Breast. Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Decorin by Simple Western^TM. Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.