| 货号 | AF142-SP |
| 别名 | DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy | 全称 | Decoy Receptor 3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human DcR3/TNFRSF6B in ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, rhTRAIL R3, and rhTRAIL R4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize DcR3/TNFRSF6B-mediated inhibition of apoptosis in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 50 ng/mL Recombinant Human DcR3/TNFRSF6B Fc Chimera, 2 ng/mL Recombinant Human Fas Ligand/TNFSF6. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8771 (Human); 107126607 (Cynomolgus Monkey) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential effect of IL-4 and IL-13 on the expression of recombination-activating genes in mature B cells from human peripheral blood. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DcR3/TNFRSF6B Val24-His300 Accession # O95407 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6, and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNF RII (29%), and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2, and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Over-expression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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DcR3/TNFRSF6B in Human Stomach Cancer Tissue. DcR3/TNFRSF6B was detected in immersion fixed paraffin-embedded sections of human stomach cancer using 10 µg/mL Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the membrane of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142). The ND50 is typically 0.1-0.5 µg/mL. |
