Human B7-2/CD86 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human B7-2/CD86 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-141-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-141-SP
别名	Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86
反应种属	Human
应用	Western Blot,Immunohistochemistry,Knockout Validated,Neutralization
目标/特异性	Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse B7-2 and recombinant rat B7-2 is observed.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL Knockout Validated: B7-2/CD86 is specifically detected in Ramos human Burkitts lymphoma parental cell line but is not detectable in B7-2/CD86 knockout Ramos cell line Neutralization: Measured by its ability to neutralize B7‑2/CD86-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. Linsley, P. et al. (1990) Proc. Natl. Acad. Sci. 87:5031. The Neutralization Dose (ND₅₀) is typically 0.25-1.25 µg/mL in the presence of 2 µg/mL Recombinant Human B7‑2/CD86 Fc Chimera and 10 µg/mL PHA.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma Authors: P Ai, Z Li, Y Jiang, C Song, L Zhang, H Hu, T Wang Oncol Lett, 2017;14(2):2458-2462. Species: Human Sample Type: Whole Tissue Application: IHC paraffin embedded Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis. Authors: Mansfield AS, Holtan SG, Grotz TE Mod. Pathol., 2011;24(4):487-94. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Modulation of T-cell activation by malignant melanoma initiating cells. Authors: Schatton T, Schutte U, Frank NY, Zhan Q, Hoerning A, Robles SC, Zhou J, Hodi FS, Spagnoli GC, Murphy GF, Frank MH Cancer Res., 2010;70(2):697-708. Species: Human Sample Type: Whole Tissue Application: IF The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. Authors: Sakthivel P, Shively V, Kakoulidou M J. Intern. Med., 2007;261(4):399-407. Species: Human Sample Type: Plasma Application: ELISA Development Human plasma contains a soluble form of CD86 which is present at elevated levels in some leukaemia patients. Authors: Hock BD, Patton WN, Budhia S, Mannari D, Roberts P, McKenzie JL Leukemia, 2002;16(5):865-73. Species: Human Sample Type: Plasma Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86 Ala23-His244 Accession # P42081
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
运输条件	Blue Ice
存放说明	4℃
参考文献	Azuma, M.et al. (1993) Nature 366:76. Freeman, G.J.et al. (1993) Science 262:909. Freeman, G.et al. (1991) J. Exp. Med. 174:625. Selvakumar, A.et al. (1993) Immunogenetics 38:292. Chen, C.et al. (1994) J. Immunol. 152:4929. Freeman, G.J.et al. (1993) J. Exp. Med. 178:2185.

参考图片

Detection of Human B7‑2/CD86 by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human B7‑2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7‑2/CD86 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

B7‑2/CD86 in Human Tonsil.
B7‑2/CD86 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7‑2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

B7‑2/CD86 in Human Tonsil.
B7‑2/CD86 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti‑Human B7‑2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑141‑NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

IL‑2 secretion Induced by
B7‑2/CD86 and Neutralization by Human B7‑2/CD86 Antibody. Recombinant Human B7‑2/CD86 Fc Chimera (Catalog # 141-B2) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Human B7‑2/CD86 Fc Chimera (2 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human B7‑2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA). The ND₅₀ is typically
0.25‑1.25 µg/mL.

Western Blot Shows Human B7‑2/CD86 Specificity by Using Knockout Cell Line. Western blot shows lysates of Ramos human Burkitts lymphoma parental cell line and B7-2/CD86 knockout Ramos cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human B7‑2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7‑2/CD86 at approximately 74 kDa (as indicated) in the parental Ramos cell line, but is not detectable in knockout Ramos cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.