Human B7-1/CD80 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human B7-1/CD80 Affinity Purified Polyclonal Ab (25 UG)

货号： AF140-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF140-SP
别名	Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human B7-1/CD80 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human B7-2 and recombinant mouse B7-2 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis. Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ Am. J. Pathol., 2011;178(2):744-53. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Urinary CD80 is elevated in minimal change disease but not in focal segmental glomerulosclerosis. Authors: Garin EH, Mu W, Arthur JM Kidney Int., 2010;78(3):296-302. Species: Human Sample Type: unrine Application: WB HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis. Authors: Kuhne M, Erben U, Schulze-Tanzil G, Kohler D, Wu P, Richter FJ, John T, Radbruch A, Sieper J, Appel H Arthritis Rheum., 2009;60(6):1635-46. Species: Human Sample Type: Whole Cells Application: Flow The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. Authors: Sakthivel P, Shively V, Kakoulidou M J. Intern. Med., 2007;261(4):399-407. Species: Human Sample Type: Plasma Application: ELISA Development Induction of B7-1 in podocytes is associated with nephrotic syndrome. Authors: Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P J. Clin. Invest., 2004;113(10):1390-7. Species: Human Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80 Extracellular domain
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through IFN-gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% amino acid identity. Human and mouse B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form theB7-1/B7-2/CD28/CTLA-4 critical binding sites.
运输条件	Blue Ice
存放说明	4℃
参考文献	Azuma, M.et al. (1993) Nature 366:76. Freeman, G.J.et al. (1993) Science 262:909. Freeman, G.et al. (1991) J. Exp. Med. 174:625. Selvakumar, A.et al. (1993) Immunogenetics 38:292. Chen, C.et al. (1994) J. Immunol. 152:4929. Freeman, G.J.et al. (1993) J. Exp. Med. 178:2185.

参考图片

Detection of Human B7‑1/CD80 by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for B7‑1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

B7‑1/CD80 in Human Tonsil. B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.