Human TSLP Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TSLP Affinity Purified Polyclonal Ab (25 UG)

货号： AF1398-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1398-SP
别名	thymic stromal lymphopoietin
全称	Thymic Stromal Lymphopoietin
反应种属	Human
应用	Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human TSLP in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse TSLP and recombinant human TSLP R is observed.
使用方法	Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 0.5 ng/mL Recombinant Human TSLP.
来源	Polyclonal Sheep IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	85480 (Human); 53603 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin. Authors: Schuepbach-Mallepell S, Philippe V, Bruggen M, Watanabe H, Roques S, Baldeschi C, Gaide O J Allergy Clin Immunol, 2013;132(6):1348-57. Species: Mouse Sample Type: tissue Application: IHC Frozen Thymic stromal lymphopoietin is produced by dendritic cells. Authors: Kashyap M, Rochman Y, Spolski R J. Immunol., 2011;187(3):1207-11. Species: Human Sample Type: Cell Lysates Application: WB Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Blood, 2010;116(12):2061-9. Species: Human Sample Type: Cell Lysates Application: WB A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs. Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A Arthritis Rheum., 2008;58(11):3510-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Constitutive and inducible thymic stromal lymphopoietin expression in human airway smooth muscle cells: role in chronic obstructive pulmonary disease. Authors: Zhang K, Shan L, Rahman MS, Unruh H, Halayko AJ, Gounni AS Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(2):L375-82. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human TSLP Tyr29-Gln159 Accession # Q969D9
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220⁺/IgM^-pre-B cells, mouse TSLP promotes the development B220⁺/IgM⁺ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c⁺ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis, and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma _c.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation Cytokines and Receptors in Allergy and Asthma Group 2 ILCs Multiple Sclerosis and Myelin Related Molecules Regulatory T Cells (Tregs) Th2 Cells TSLP, Inflammation, & Cancer

参考图片

Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody. Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND₅₀ is typically 0.05-0.25 µg/mL.