Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
Neuron, 2009;62(4):510-25.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen
Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma.
Authors: Hu J, Dong A, Fernandez-Ruiz V, Shan J, Kawa M, Martinez-Anso E, Prieto J, Qian C
Cancer Res., 2009;69(17):6951-9.
Species: Human
Sample Type: Tissue Homogenates
Application: WB
Wnt signaling antagonists are potential prognostic biomarkers for the progression of radiographic hip osteoarthritis in elderly Caucasian women.
Authors: Lane NE, Nevitt MC, Lui LY, de Leon P, Corr M
Arthritis Rheum., 2007;56(10):3319-25.
Species: Human
Sample Type: Serum
Application: ELISA Development
Genomics identifies medulloblastoma subgroups that are enriched for specific genetic alterations.
Authors: Thompson MC, Fuller C, Hogg TL, Dalton J, Finkelstein D, Lau CC, Chintagumpala M, Adesina A, Ashley DM, Kellie SJ, Taylor MD, Curran T, Gajjar A, Gilbertson RJ
J. Clin. Oncol., 2006;24(12):1924-31.
Species: Human
Sample Type: Whole Tissue
Application: IHC

Secreted Frizzled Related Proteins (sFRPs) are a family of secreted, soluble vertebrate glycoproteins which contain homology to the Wnt-binding domain of the Frizzled (Fz) family of transmembrane receptors. sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; an N-terminal Fz cysteine-rich domain (CRD) with 10 conserved cysteines; and a C-terminal heparin-binding region with weak homology to Netrin. The Fz CRD mediates Wnt-binding and is present in all Fz and sFRP family members (1).

sFRP-1, also known as secreted apoptosis-related protein 2 (SARP-2), FRP and FrzA, is expressed in the embryonic kidney, eye, brain, teeth, salivary gland and small intestine, most often at sites of epithelial-mesenchyme interaction (5). Expression in the adult animal is strong in the eye, kidney, and heart and also prevalent in the brain and lung (2, 5). sFRP-1 was first characterized as a protein that enhances the sensitivity of cells to apoptotic stimuli (3) and as an antagonist of Wnt signaling in Xenopus embryos (4). It is also characterized as a tumor suppressor in breast (6) and cervical carcinomas (7). In contrast, sFRP-1 is expressed by the majority of malignant gliomas (8) and contributes to the development of uterine leiomyomas (9), suggesting that the role of sFRP-1 is dependent on cell context. sFRP-1 has diverse activities, from inducing angiogenesis (10) in a variety of in vivo models to helping regulate Wnt-4 signaling (with sFRP-2) in renal organogenesis (11). Mouse and human sFRP-1 proteins share 94% amino acid identity (1).

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2. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
3. Melkonyan, H. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13636.
4. Finch, P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
5. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
6. Ugolini, F. et al. (2001) Oncogene 20:5810.
7. Ko, J. et al. (2002) Exp. Cell. Res. 280:280.
8. Roth, W. et al. (2000) Oncogene 19:4210.
9. Fukuhara, K, et al. (2002) J. Clin. Endocr. Metab. 87:1729.
10. Dufourcq, P. et al. (2002) Circulation 106: 3097.
11. Yoshino, K. et al. (2001) Mech. Dev. 102:45.