Human P-Selectin/CD62P Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human P-Selectin/CD62P Affinity Purified Polyclonal Ab (25 UG)

货号： AF137-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF137-SP
别名	CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; selectin P (granule membrane protein 140kDa, antigen CD62); SELP
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human P-Selectin/CD62P in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse P-Selectin and recombinant rat P-Selectin is observed, and less than 1% cross-reactivity with recombinant human (rh) E-Selectin and rhL-Selectin is observed.
使用方法	Western Blot: 1 µg/mL Adhesion Blockade: The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized recombinant human P‑Selectin (10 μg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 μg/mL of the antibody.
来源	Polyclonal Sheep IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6403 (Human); 20344 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G ALTEX, 2011;28(4):305-16. Species: Human Sample Type: Cell Lysates Application: WB The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. Authors: Kato N, Yuzawa Y, Kosugi T J. Am. Soc. Nephrol., 2009;20(7):1565-76. Species: Human Sample Type: Whole Cells Application: Neut Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy. Authors: Choudhury A, Chung I, Blann AD, Lip GY Chest, 2007;131(3):809-15. Species: Human Sample Type: Plasma Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human P‑Selectin/CD62P
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.
运输条件	Blue Ice
存放说明	4℃
参考文献	Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Human CD Marker Products ICC / IHC Images: P-Selectin/CD62P Lectins Mini-review: Adhesion Molecules I Mini-review: Adhesion Molecules II Mini-review: Multiple Sclerosis: Immune Cell Access to the Central Nervous System Platelets Regulatory T Cells (Tregs) Selectins

参考图片

Detection of Human P‑Selectin/CD62P by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human
P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for P‑Selectin/
CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.