| 货号 | AF13662-SP |
| 别名 | EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1 | 全称 | Protein Tyrosine Phosphatase 1B |
| 反应种属 | Human |
| 应用 | Western Blot(0.3 µg/mL) |
| 目标/特异性 | Detects endogenous human PTP1B and does not cross-react with recombinant mouse PTP1B in Western blots. |
| 使用方法 | Western Blot: 0.3 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5770 (Human); 19246 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PTP1B Glu2-Thr435 Accession # P18031 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PTP1B by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CHP‑100 human neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
