Human/Mouse/Rat p53 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat p53 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1355-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1355-SP
别名	Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
反应种属	Human/Mouse/Rat
应用	Western Blot,Simple Western,Immunoprecipitation,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Knockout Validated
目标/特异性	Detects human, mouse, and rat p53.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunoprecipitation: 1-2 µg/500 µg cell lysate Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha. Authors: Tsoporis JN, Izhar S, Parker TG J. Biol. Chem., 2008;283(44):30174-83. Species: Rat Sample Type: Cell Lysates Application: WB Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon. Authors: Ishida T, Sakaguchi I, Biol. Pharm. Bull., 2007;30(5):928-34. Species: Human Sample Type: Cell Lysates Application: WB TIMP-1 gene deficiency increases tumour cell sensitivity to chemotherapy-induced apoptosis. Authors: Davidsen ML, Wurtz SÃƒËœ, Romer MU, Sorensen NM, Johansen SK, Christensen IJ, Larsen JK, Offenberg H, Brunner N, Lademann U Br. J. Cancer, 2006;95(8):1114-20. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human p53 Asp7-Asp393 Accession # P04637
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Transcription Factors and Regulators Cancer Biomarkers Cancer Stem Cell Transcription Factors Cell Cycle Checkpoint DNA Damage and Repair Epithelial Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: p53 Immunoglobulin-Like Domain Transcription Factors Mesenchymal Stem Cell Transcription Factors Mini-review: DNA Damage Response Mini-review: Genomic Instability Syndromes Neural Stem Cell Transcription Factors p53 Pathway p53: More than a Transcription Factor? Parkinsons Disease Related Molecules Pluripotent Stem Cell Transcription Factors TNF Superfamily Regulators Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of p53-regulated Genes by Chromatin Immunoprecipitation. MCF‑7 human breast cancer cell line treated with 300 nM camptothecin overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. p53/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.

p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human p53 by Simple Western^TM. Simple Western lane view shows lysates of CEM human
T-lymphoblastoid cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p53 at approximately 59 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.