| 货号 | AF1350-SP |
| 别名 | CD49 antigen-like family member F; CD49f antigen; CD49f; DKFZp686J01244; FLJ18737; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B; Platelet gpl; VLA-6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human Integrin alpha 6/CD49f in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 9 is observed and less than 1% cross-reactivity with rhIntegrin alpha 2, recombinant mouse (rm) Integrin alpha 3, rhIntegrin alpha 7, and rmIntegrin alpha 7 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3655 (Human); 16403 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Beta4 integrin promotes osteosarcoma metastasis and interacts with ezrin. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Integrin alpha 6/CD49f Pro93-Arg148, Ile557-Arg613 Accession # P23229 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130‑150 kDa member of the integrin alpha chain family of molecules. Mature Integrin alpha 6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24‑938), and a 30‑31 kDa membrane-bound light chain (aa 942‑1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin. Splice variants exist that show either a deletion of aa 215‑258 or a deletion of aa 260‑298, possibly accompanied by a 29 aa substitution for aa 1084‑1130. Over aa 93‑148 and 518‑574, human Integrin alpha 6 shares 89% aa identity with mouse Integrin alpha 6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Integrin alpha 6/CD49f by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin alpha 6/CD49f at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Human Integrin alpha 6/CD49f by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue (cortex), loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 6/CD49f at approximately 167 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Integrin alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
