Human ULBP-2/5/6 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human ULBP-2/5/6 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1298-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1298-SP
别名	ULBP-2/5/6
全称	UL16 Binding Protein-2/5/6
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human ULBP-2, ULBP-5, and RAET1L/ULBP-6 in Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) ULBP-1, rhULBP-3, and rhULBP-4 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 25 ng/mL of biotinylated Recombinant Human ULBP-2 Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding. This antibody will block >90% of the binding of either Recombinant Human ULBP-5 Fc Chimera or Recombinant ULBP-6 Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60. Authors: Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W J Immunol, 2015;194(6):2930-41. Species: Human Sample Type: Cell Lysates Application: WB MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. Authors: Cho, Hanbyoul, Chung, Joon-Yon, Kim, Sunghoon, Braunschweig, Till, Kang, Tae Heun, Kim, Jennie, Chung, Eun Joo, Hewitt, Stephen, Kim, Jae-Hoon BMC Cancer, 2014;14(0):957. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D. Authors: Heinemann A, Zhao F, Pechlivanis S, Eberle J, Steinle A, Diederichs S, Schadendorf D, Paschen A Cancer Res., 2012;72(2):460-71. Species: Human Sample Type: Tissue Homogenates Application: WB The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells. Authors: Fernandez-Messina L, Ashiru O, Aguera-Gonzalez S, Reyburn HT, Vales-Gomez M J. Cell. Sci., 2011;124(0):321-7. Species: Human Sample Type: Whole Cells Application: ICC Secretome-Based Identification of ULBP2 as a Novel Serum Marker for Pancreatic Cancer Detection. Authors: Chang YT, Wu CC, Shyr YM, Chen TC, Hwang TL, Yeh TS, Chang KP, Liu HP, Liu YL, Tsai MH, Chang YS, Yu JS PLoS ONE, 2011;6(5):e20029. Species: Human Sample Type: Cell Lysates Application: WB Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform. Authors: Ohashi M, Eagle RA, Trowsdale J J. Biol. Chem., 2010;285(22):16408-15. Species: Human Sample Type: Cell Lysates Application: WB Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Authors: Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A Int. J. Cancer, 2009;124(7):1594-604. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human ULBP-2 Gly26-Ser217 Accession # Q9BZM5
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cosman, D. et al. (2001) Immunity 14:123. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428. Sutherland, C. et al. (2002) J. Immunol. 168:671. Steinle, A. et al. (2001) Immunogenetics 53:279. Sutherland, C. et al. (2001) Immunol. Rev. 181:185. Pende, D. et al. (2002) Cancer Res. 62:6178. Radosavljevic, M. et al. (2002) Genomics 79:114. NKG2D and its Ligands (2002) www.RnDSystems.com.

参考图片

ULBP‑2/5/6 in Human Colon Cancer Tissue. ULBP‑2/5/6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 10 µg/mL Goat Anti-Human ULBP‑2/5/6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1298) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.