Human TREM-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TREM-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1278-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1278-SP
别名	CD354 antigen; CD354; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
全称	Triggering Receptor Expressed on Myeloid Cells 1
反应种属	Human
应用	Western Blot,Flow Cytometry,Agonist Activity,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human TREM-1 in ELISAs and Western blots. In ELISAs, less than 0.2% cross-reactivity with recombinant mouse (rm) TREM‑1 and rmTREM‑2b is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Agonist Activity: Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells. Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991. The ED₅₀ for this effect is typically 2 - 6 μg/mL. ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	54210 (Human); 58217 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes. Authors: Gomez-Pina V, Soares-Schanoski A, Rodriguez-Rojas A, Del Fresno C, Garcia F, Vallejo-Cremades MT, Fernandez-Ruiz I, Arnalich F, Fuentes-Prior P, Lopez-Collazo E J. Immunol., 2007;179(6):4065-73. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1 Met1-Arg200 Accession # Q9NP99
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells. Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991. The ED₅₀ for this effect is typically 2 - 6 μg/mL.
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Bouchon, A. (2000) J. Immunol. 164:4991. Bouchon, A. (2001) Nature 410:1103. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

参考图片

Detection of Human TREM‑1 by Western Blot. Western blot shows lysates of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TREM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.