Human Jagged 1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Jagged 1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1277-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1277-SP
别名	AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; jagged 1; Jagged1; JAGL1; protein jagged-1
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat Jagged 1 is observed and less than 1% cross-reactivity with recombinant human Jagged 2 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Jagged 1-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Human Jagged 1 Fc Chimera.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	182 (Human); 29146 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin-6 Stimulates Defective Angiogenesis. Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F Cancer Res, 2015;75(15):3098-107. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR Cancer Res., 2012;72(1):66-75. Species: Human Sample Type: Cell Lysates Application: WB Interleukin-6 as a therapeutic target in human ovarian cancer. Authors: Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirstrom K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR Clin. Cancer Res., 2011;17(18):6083-96. Species: Human Sample Type: Cell Lysates Application: WB Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping. Authors: Roesli C, Mumprecht V, Neri D, Detmar M FASEB J., 2008;22(6):1933-44. Species: Human Sample Type: Cell Lysates Application: WB Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F Stem Cells, 2007;26(1):279-89. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Jagged 1 Ser32-Asp296 Accession # P78504
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849. Artavamis-Tsokanas, S. et al. (1999) Science 284:770. Lai, E.C. (2004) Development 131:965. Milner, L.A. et al. (1994) Blood 83:2057. Nyfeler, Y. et al. (2005) EMBO J. 24:3504. Linheng, L. et al. (1998) Immunity 8:43. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543. Oda, T. et al. (1997) Nat. Genet. 16:235. Oda, T. et al. (1997) Genomics 43:376.

参考图片

Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh‑7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND₅₀ is typically 1-5 µg/mL.