Human SLPI Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human SLPI Affinity Purified Polyclonal Ab (25 UG)

货号： AF1274-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1274-SP
别名	ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI
全称	Secretory Leukocyte Protease Inhibitor
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with mouse SLPI is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human SLPI (0.125 µg/mL, Catalog # 1274-PI) inhibition of Trypsin (0.5 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (10 µM, Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 1.5 µg/mL.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6590 (Human); 20568 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Secretory leukocyte protease inhibitor (SLPI) expression and tumor invasion in oral squamous cell carcinoma. Authors: Wen J, Nikitakis NG, Chaisuparat R, Greenwell-Wild T, Gliozzi M, Jin W, Adli A, Moutsopoulos N, Wu T, Warburton G, Wahl SM Am. J. Pathol., 2011;178(6):2866-78. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients. Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T Mol. Cell Proteomics, 2008;7(10):1974-82. Species: Human Sample Type: Serum Application: immunoaffinity-mass spectromet The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils. Authors: Jacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K J. Leukoc. Biol., 2008;83(5):1155-64. Species: Human Sample Type: Cell Culture Supernates Application: WB The major bactericidal activity of human seminal plasma is zinc-dependent and derived from fragmentation of the semenogelins. Authors: Edstrom AM, Malm J, Frohm B, Martellini JA, Giwercman A, Morgelin M, Cole AM, Sorensen OE J. Immunol., 2008;181(5):3413-21. Species: Human Sample Type: seminal plasma Application: WB The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cells. Authors: Simpkins FA, Devoogdt NM, Rasool N, Tchabo NE, Alejandro EU, Kamrava MM, Kohn EC Carcinogenesis, 2007;29(3):466-72. Species: Human Sample Type: Cell Lysates Application: WB cDNA microarray analysis of invasive and tumorigenic phenotypes in a breast cancer model. Authors: Kluger HM, Kluger Y, Gilmore-Hebert M, DiVito K, Chang JT, Rodov S, Mironenko O, Kacinski BM, Perkins AS, Sapi E Lab. Invest., 2004;84(3):320-31. Species: Human Sample Type: Whole Cells Application: ICC Antimicrobial components of vaginal fluid. Authors: Valore EV, Park CH, Igreti SL, Ganz T Am. J. Obstet. Gynecol., 2002;187(3):561-8. Species: Human Sample Type: Vaginal Fluid Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human SLPI
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).
运输条件	Blue Ice
存放说明	4℃
参考文献	Zhang, D. et al. (2002) J. Biol. Chem. 277:29999. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

参考图片

SLPI in Human Lung.

SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1274) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.